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Free of charge guidance in sample preparation and panel design, and affordable prices for instrument. A meeting with both Flow Cytometry and Genomics Core directors is mandatory for pilot 10x experiments. In addition, the Core frequently organizes training seminars for various levels of expertise. Materials available for reference in the Flow CytometryĬore lab include two introductory tutorials available in iLab, several textbooks on the basics of flow cytometry and the current protocols in cytometry. Core technologists are available forĪssistance with sample acquisition, analysis and presentation. Protocols for staining, fixation and data analysis methods. Potential problems can be averted before critical resources are used. This meeting is often useful in that many Consultation:Īll investigators are encouraged to meet with the Scientific Director of the Flow Cytometry Core to discuss their experiment prior to ordering antibodies and other reagents. #Export to pdf not working flowjo 10 software
SpectroFlo, FACSDIVA and FACSChorus are for acquisition only.Īnalysis workstations and software training are available for researchers.
Histogram Software (Apogee Flow Systems). The Core provides data analysis with the following software: The ID7000 is ideal for analyzing samples with complex heterogeneous cell populations. The instrument has six lasers and 182 detectors for complete spectral data acquisition, allowing up to +45 color panels, load-and-walkaway data acquisition and advanced autofluorescence subtraction tool. Sony ID7000 spectral flow cytometer with advanced high-parameter flow cytometry capabilities. Samples can easily be acquired from 96 well plate format or single tube mode. Cytek Aurora spectral flow cytometer equipped with five lasers (355nm, 405nm, 488nm, 561nm and 640 nm excitation wavelengths), three scattering channels, can detect up to 64 fluorescence channels. 10x Chromium Controller for single cell partitioning for transcriptome analysis. Personnel, product, and environmental protection from potentially aerosolized biological hazards. All cell sorters are housed in specialty type Class II biological safety cabinets designed to offer. 
Becton-Dickinson FACS Melody cell sorter (488nm, 561nm, and 640nm direct diode.The BD SORP FACSAria Fusion cell sorter consists of 355nm, 405nm, 488nm, 561nm, and 637nm lasers, providing 20 parameters. Becton-Dickinson SORP FACSAria Fusion high performance with ACDU, fixed alignment gel-coupled cuvette cell sorter.
Becton-Dickinson High Throughput Sampler (HTS) capable of acquiring samples from 96-well orģ84-well microtiter plates on the LSRFortessa.Becton-Dickinson LSRFortessa capable of 18 color and 20 parameter acquisition with 5 lasers (355nm, 407nm, 488nm, 561nm, and 641nm).Zetaview Nanoparticle Tracking Analyzer.Apogee A50 Micro used for enumeration and fluorescence of small particle applications.
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